Mapping the Pax6 3’ untranslated region microRNA regulatory landscape

The ODC 3′-Untranslated Region and 5′-Untranslated Region Contain cis-Regulatory Elements: Implications for Carcinogenesis

It has been hypothesized that both the 3'-untranslated region (3'UTR) and the 5'-untranslated region (5'UTR) of the ornithine decarboxylase (ODC) mRNA influence the expression of the ODC protein. Here, we use luciferase expression constructs to examine the influence of both UTRs in keratinocyte derived cell lines. The ODC 5'UTR or 3'UTR was cloned into the pGL3 control vector upstream or downst...

Expression of Versican 3′-Untranslated Region Modulates Endogenous MicroRNA Functions

BACKGROUND Mature microRNAs (miRNAs) are single-stranded RNAs that regulate post-transcriptional gene expression. In our previous study, we have shown that versican 3'UTR, a fragment of non-coding transcript, has the ability to antagonize miR-199a-3p function thereby regulating expression of the matrix proteins versican and fibronectin, and thus resulting in enhanced cell-cell adhesion and orga...

Mapping the Shh regulatory landscape

Biological basis for restriction of microRNA targets to the 3¢ untranslated region in mammalian mRNAs

MicroRNAs (miRNAs) interact with target sites located in the 3¢ untranslated regions (3¢ UTRs) of mRNAs to downregulate their expression when the appropriate miRNA is bound to target mRNA. To establish the functional importance of target-site localization in the 3¢ UTR, we modified the stop codon to extend the coding region of the transgene reporter through the miRNA target sequence. As a resul...

Regulatory 3′ Untranslated Regions of Bacterial mRNAs

The untranslated regions (UTRs) of mRNA contain important features that are relevant to the post-transcriptional and translational regulation of gene expression. Most studies of bacterial UTRs have focused on the 5'regions; however, 3'UTRs have recently emerged as a new class of post-transcriptional regulatory elements. 3'UTRs were found to regulate the decay and translation initiation in their...